ABSTRACT
BACKGROUND: Ralstonia solanacearum causes bacterial wilt of Pogostemon cablin which is an important aromatic herb and also the main materials of COVID-19 therapeutic traditional drugs. However, we are lacking the information on the genomic sequences of R. solanacearum isolated from P. cablin. OBJECTIVE: The acquisition and analysis of this whole-genome sequence of the P. cablin bacterial wilt pathogen. METHODS: An R. solanacearum strain, named SY1, was isolated from infected P. cablin plants, and the complete genome sequence was sequenced and analyzed. RESULTS: The SY1 strain contains a 3.70-Mb chromosome and a 2.18-Mb megaplasmid, with GC contents of 67.57% and 67.41%, respectively. A total of 3308 predicted genes were located on the chromosome and 1657 genes were located in the megaplasmid. SY1 strain has 273 unique genes compared with five representative R. solanacearum strains, and these genes were enriched in the plant-pathogen interaction pathway. SY1 possessed a higher syntenic relationship with phylotype I strains, and the arsenal of type III effectors predicted in SY1 were also more closely related to those of phylotype I strains. SY1 contained 14 and 5 genomic islands in its chromosome and megaplasmid, respectively, and two prophage sequences in its chromosome. In addition, 215 and 130 genes were annotated as carbohydrate-active enzymes and antibiotic resistance genes, respectively. CONCLUSION: This is the first genome-scale assembly and annotation for R. solanacearum which isolated from infected P. cablin plants. The arsenal of virulence and antibiotic resistance may as the determinants in SY1 for infection of P. cablin plants.
ABSTRACT
The hospitality work environment presents many unique challenges for employees and organizations, such as the intertwined and collective work nature, implicit job expectations, and a shrinking labor market. The demand for highly skilled employees who are sufficient to deliver the unique brand values to customers is on the rise for building successful service brands through employees. The need to retain talented employees is further intensified by the "great resignation” movement in the USA along with the COVID-19 pandemic. This study demonstrates the positive role of brand-specific transformational leadership in promoting employees' highly engaged brand building behavior (investment-of-self) and their resistance to outside competing job offers. More importantly, drawing upon cognitive dissonance theory, this study shows that the impact of brand-specific transformational leadership is mediated by employees' sense of brand community internally, as well as moderated by perceived brand promise accuracy. These results supported the essential role of achieving employees' cognitive consonance in brand communication both internally and externally. These results are supported by 203 US hospitality employee responses from multiple data collections purposefully designed with temporal and cognitive distance.